Acyl-CoA synthetases as regulators of brain phospholipid acyl-chain diversity

Giardia fatty acyl-CoA synthetases as potential drug targets

Giardiasis caused by Giardia intestinalis (syn. G. lamblia, G. duodenalis) is one of the leading causes of diarrheal parasitic diseases worldwide. Although limited drugs to treat giardiasis are available, there are concerns regarding toxicity in some patients and the emerging drug resistance. By data-mining genome sequences, we observed that G. intestinalis is incapable of synthesizing fatty ac...

Versatility of acyl-acyl carrier protein synthetases.

The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...

Acyl-CoA dehydrogenases and acyl-CoA oxidases

Acyl-CoA dehydrogenases and acyl-CoA oxidases are two closely related FAD-containing enzyme families that are present inmitochondria andperoxisomes, respectively. They catalyze the dehydrogenation of acyl-CoA thioesters to the corresponding trans-2-enoyl-CoA. This review examines the structure of medium chain acyl-CoA dehydrogenase, as a representative of the dehydrogenase family, with respect ...

[Very-long-chain acyl-CoA dehydrogenase deficiency].

Short-chain acyl-CoA dehydrogenase deficiency.

The c.625G>A variant of the short-chain acyl-CoA dehydrogenase (SCAD) gene is considered to confer susceptibility for developing “clinical SCAD deficiency (SCADD)” and appears to be common in the general population. To determine the frequency of the c.625G>A variant in the Netherlands, we analyzed 1036 screening cards of 5to 8-dayold newborns and found 5.5% homozygous and 31.3% heterozygous for...